BAFF CHO
CBP74286
| I. Background | |
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BAFF（B 细胞激活因子，B Cell Activating Factor），也称为 TNFSF13B（Tumor Necrosis Factor Superfamily Member 13B）或 BLyS（B Lymphocyte Stimulator），是一种属 于肿瘤坏死因子（TNF）超家族的细胞因子。BAFF 主要由单核细胞、巨噬细胞、树突状 细胞和某些基质细胞产生，在 B 细胞的存活、增殖和成熟中起关键作用。 |
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| II. Description | |
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BAFF CHO 细胞，在 CHO-K1 细胞的基础上，稳定表达人 BAFF。BAFF CHO 细胞作为 TACI Effector Reporter Cell 细胞或者 BAFFR Effector Reporter Cell 细胞的靶细胞，很好的模拟了体内 TACI&BAFF 或者 BAFFR&BAFF 的信号转导过程，原理见下图所示。
Figure 2. BAFFR&BAFF 信号转导模型原理图
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| III. Introduction | |
| Expressed gene: | BAFF |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+600 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
IV. Representative Data Figure 3. Recombinant BAFF CHO stably expressing BAFF.  Figure 4. Blocking of BAFF induced TACI Effector Reporter Cell(C32) Activity by BAFF Neutralization Ab with BAFF CHO. |
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 Figure 5. Blocking of BAFF induced BAFFR Effector Reporter Cell(C14) Activity by BAFFR Blocking Ab with BAFF CHO. Blocking of BAFF induced BAFFR Effector Reporter Cell(C14) Activity by BAFFR Blocking Ab with BAFF CHO. |
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